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Interaction of Plasmid DNA (pBR322) with D-ribose

Faculty Supervisor:
Gary Histand
Year of Project Completion:
Franklin Ekue


In the past, research has been carried out to find out the effects reducing sugars have with nucleic acids such as DNA. When these researches were carried out, there were several interesting results and some of them are of particular interest. They are DNA strand breakage and DNA modification. These two results are of particular interest because of the fact that aging and age – related dysfunction in gene expression are important biological processes and some of the causes of the above biological processes are DNA strand breakage and DNA modification. So, some reducing sugars such as glucose and fructose have been tested to produce the above effects. In this research project, the reducing sugar used was D-ribose and the research project was about trying to see if D-ribose produces similar effects when reacted with the plasmid pBR322, as seen with other tested reducing sugars (glucosamine) when reacted with DNA. Three parameters were varied; buffer type, presence of different metal ions, and ribose concentration. At the end of each experiment, agarose gel electrophoresis and then a computer program called TNImage were used to analyze the reaction contents.

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As the first Mennonite college founded in North America, Bethel College celebrates a tradition of progressive Christian liberal arts education, diversity within community, and lifelong learning.